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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.


Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

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Cleaning

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One of the tissue culture incubators has a birthday.
25th Nov 2011, 07:12   comments (3)

Wild western

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Well, western blotting anyway. Ponceau staining says the protein has separated out nicely on the gel and has transfered to the membrane OK. A couple of small air bubbles letting me down but nothing major. Now it just needs incubating with primary antibody, washing, incubating with secondary antibody (linked to a detection substance), then detection to see what's where and if there's anything interesting going on.

This is the pretty bit :)
*edit* Unfortunately the camera doesn't like to focus on it for some reason. It's a lot clearer to the naked eye, honest!
27th Oct 2011, 23:18   | tags:,comments (0)

All grown up

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Well, nearly. They've grown as much as they are likely to for this year and will soon go to their winter place for a well deserved dormancy. To think in July these were all mere twinkles in the eye of pieces of root.
27th Oct 2011, 23:04   comments (0)

See instars

This week I have been mostly babysitting these hungry little red poplar leaf beetle larvae (Chrysomela populi). And when I say hungry, I mean really, really hungry! They go through a dramatic series of moults before finally pupating and turning into the cute, speedy (also hungry) adults. This lot haven't got that far yet, but hopefully I'll see a few of the later stages of development too.
17th Sep 2011, 20:21   comments (8)

Hardening off

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Toughening up for winter :)
22nd Aug 2011, 22:52   comments (1)

Roots! Or shoots?

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Something is happening to the aspen cuttings. I'm hopeful it's roots and not more attempts to send up shoots. A little longer to be sure...
22nd Aug 2011, 22:47   comments (0)

Trying a different way

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To see if aspen root cuttings will root in plain old tap water, and to see if they root faster than in soil.
17th Aug 2011, 22:33   comments (0)

rootlessness in the early days

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There has been a somewhat high casualty rate to begin with for my poor little aspens, but I think we have the conditions under more control now. I have high hopes for these little guys.
17th Aug 2011, 22:25   comments (1)
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