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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.


Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

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Little aspens...

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:)
31st Jul 2011, 12:17   | tags:comments (4)

Getting to the root of the matter

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Aspen roots, that is.
31st Jul 2011, 12:11   comments (0)

It's cake, Jim...

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but not as we know it.

Today's experiment is kitchen based and involves muffins and savory stuff.
20th Jul 2011, 16:14   comments (8)

And now for something completely different

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Not the larch but the aspen :)
My first day doing horticulture has been spent mostly planting up aspen roots in an attempt to propagate more trees. It makes a nice change from my usual more human-biased molecular biology. It's going to be interesting being a part-time plant7/part-time animal person, for me at least.
14th Jul 2011, 22:45   comments (2)

Oh ELISA

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It's been a while since my last post here, but I'm still doing science where I can. For the last few months I've been volunteering in an MS research lab where I have been mostly running ELISAs - assays that use protein-specific antibodies to measure levels of your favourite protein of the day in your samples, in this case neurofilament light-chain levels in cersbrospinal fluid samples. It's good to keep my hand in and learn some new stuff while I try to find a proper job. I guess you never know where things will lead.
14th Jul 2011, 22:37   comments (5)

The unhappiest PCR in the world (so far)

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With a lot of persuasion the enzyme is just about making the band that I want it too. You can hardly see it, but it is there in the middle 4 lanes. The lanes at either end are ladders of size markers (Pieces of DNA at different sizes). The band I want is just below the position of the second band from the top on the left-hand ladder, directly below the dark circles left by the loading dye (loading dye helps you see a) that you have got your DNA into the gel in the first place and b) how far your DNA has run when the gel is in the tank).

I have cut out the regions where my band is and will extract the DNA from that, then use that as template for another round of PCR to amplify up that specific bit some more. That's the plan.

*edit: just looking at this posted here you really can't see the bands at all except maybe the merest hint of it in the 3rd middle lane (from left to right). You can just about see it on the original and with the naked eye (behind a visor!) and a serious UV light source.
10th Jan 2010, 22:36   comments (4)

multi-tasking

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Have lots of little projects on the go right now. Some of them working, some of them being trouble-shot (if that can be a word). My bench is a constantly changing (slightly cluttered) area of tiny tubes and larger tubes.
8th Jan 2010, 22:22   comments (4)

Happy New Cloning

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So far, so good for the new year and new cloning. These look like happy plasmids in happy bugs. All controls were perfect. Just need to test them now... see if they really have the gene I want, then I can grow up more and extract the plasmid to use in actual experiments.
5th Jan 2010, 20:10   comments (2)