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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.

Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

Recent visitors

Yes! (maybe...)

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As a last resort I went back and PCR checked all the tiny, tiny colonies that came up after about 2 weeks of incubation, just in case any of them had my piece of DNA I've been trying to get them to take up. I used primers (short pieces of single stranded DNA) that should recognise the beginning and end of the sequence and used to PCR to amplify any sequence that was there. And this is what I got. The bands are about the right size (same size as the positive control anyway (bottom right, last well). Streaked out the colonies onto a fresh plate so am hoping that they will grow some more over night. If that works I can start to bulk them up and then extract enough DNA to do an actual experiment on some real mammalian cells! An actual experiment! Feels like a long time since I've done one of them, rather than just trying to get my toolbox in order :)
Still keeping everything crossed.
27th Nov 2009, 22:45   | tags:


Caine says:

Yay! Fingers crossed. :)

28th Nov 2009, 21:19

Spidermonkey says:

:) Time will tell.

29th Nov 2009, 22:14