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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.


Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)


Recent visitors

Something is different

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This was the first test of the thing I have been so desperately trying to clone over the last few weeks (feels like forever!).
I transfected the plasmid into HuH7 (liver) cell line into some of the wells on a 12 well plate, and control plasmids into other wells. The top 2 images are from 2 different wells that got my plasmid. I was hoping that they would look like the cells in image 3 (where most of the green is in the cytoplasm, the dark ovals being the cell nuclei), so that's a shame, however they do look different to the GFP only control well (image 4), where the green is kind of diffuse and all over the cells) so something at least has changed. A lot of the cells with my plasmid have what look like clumps of very bright green, which is most likely due to the protein getting stuck somewhere in the cellular machinery where proteins are assembled.

Trying this out in a different cell line to see whether or not a different cell type will be better at processing the protein. By better, I mean, give me a phenotype more like that in the 3rd shot. If that works, then I can try treating the cells with chemical compounds known to bind to this protein and see if the fluorescence moves into the nucleus. And if that works then I will have a model I can use to test other compounds to see if they, too, will be bound by my protein and are therefore likely to have a biological effect downstream of this pathway. In other words, then I can actually do an experiment! :)
18th Dec 2009, 18:26  

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FilbertFox says:

at least gfp looks nice, so even when things don't go to plan you can go 'oh look pretty!'

22nd Dec 2009, 14:36